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Examination method: Bone marrow examination Examination process: (1) Select the puncture site (2) Anesthetize (3) Fix the length of the puncture needle (4) The doctor's left thumb and index finger fix the puncture site, and the right hand holds the bone marrow puncture needle to puncture perpendicular to the bone surface. For sternum puncture, it should be inserted at an angle of 30 degree -40 degree with the bone surface. When the needle tip of the puncture needle touches the bone, rotate the puncture needle left and right along the long axis of the needle body, and push it forward to slowly penetrate the bone. (5) Extract the bone marrow fluid, pull out the core of the puncture needle, connect a dry syringe (10m1 or 201m1), and extract the bone marrow fluid with appropriate force. Steps of bone marrow cytology examination: (1) Smear: It is required that the smear slides and push slides should be clean and free of putty contamination. use. (2) Staining: Wright-Giemsa mixed staining method is commonly used; cytochemical staining is often used in combination. (3) Low magnification microscopy: To judge the degree of bone marrow hyperplasia, the ratio of mature red blood cells to nucleated cells in bone marrow slices is usually used to judge the degree of bone marrow hyperplasia. (4) Oil microscope examination: Select a place where the cells are evenly distributed, classify and count at least 200 nucleated cells under oil microscope, and pay attention to whether there is any qualitative change.

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